2YZS
Crystal structure of uncharacterized conserved protein from Aquifex aeolicus
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-06-01 |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 0.9788, 0.9000, 0.9794 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 122.235, 96.979, 93.495 |
| Unit cell angles | 90.00, 127.34, 90.00 |
Refinement procedure
| Resolution | 40.610 - 2.000 |
| R-factor | 0.219 |
| Rwork | 0.219 |
| R-free | 0.25400 |
| Structure solution method | MAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.100 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.055 | 0.302 |
| Number of reflections | 57566 | |
| <I/σ(I)> | 15.7 | 3.8 |
| Completeness [%] | 99.1 | 97.2 |
| Redundancy | 3.6 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 1.7M Ammonium sulfate, 85MM HEPES pH7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
