2YZJ

Crystal structure of dCTP deaminase from Sulfolobus tokodaii

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B2
Synchrotron site	SPring-8
Beamline	BL26B2
Temperature [K]	100
Detector technology	CCD
Collection date	2006-12-09
Detector	RIGAKU JUPITER 210
Wavelength(s)	0.9789, 0.9794, 0.9000
Spacegroup name	P 1
Unit cell lengths	53.770, 53.757, 53.757
Unit cell angles	107.47, 107.52, 107.50

Refinement procedure

Resolution	31.650 - 1.660
R-factor	0.208
Rwork	0.208
R-free	0.22700
Structure solution method	MAD
RMSD bond length	0.009
RMSD bond angle	1.400
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.660
High resolution limit [Å]	1.600	1.600
Rmerge	0.121	0.236
Number of reflections	90293
Completeness [%]	92.0
Redundancy	2.7	2.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	0.17M Magnesium Acetate, 0.085M Sodium Cavpdylate pH 6.5, 17% PEG 8000, 0.01M dUTP, 0.01M Magnesium Chloride, 0.02M Tris-HCl pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K