2YZD

Crystal structure of uricase from Arthrobacter globiformis in complex with 8-azaxanthin (inhibitor)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	100
Detector technology	CCD
Collection date	2003-12-12
Detector	ADSC QUANTUM 210
Wavelength(s)	1.00
Spacegroup name	P 21 21 21
Unit cell lengths	83.330, 122.280, 283.920
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.240
Rwork	0.189
R-free	0.23930
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1uox
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.320
High resolution limit [Å]	2.240	2.240
Rmerge	0.067	0.237
Number of reflections	132537
Completeness [%]	97.4	87.7
Redundancy	5.4	5.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9	277	PEG 8000, lithium sulfate, sodium borate buffer, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K