2YY8

Crystal structure of archaeal tRNA-methylase for position 56 (aTrm56) from Pyrococcus horikoshii, complexed with S-adenosyl-L-methionine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	100
Detector technology	CCD
Collection date	2006-11-05
Detector	ADSC QUANTUM 210
Wavelength(s)	1.0000
Spacegroup name	P 32
Unit cell lengths	56.151, 56.151, 121.844
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	45.170 - 2.480
R-factor	0.216
Rwork	0.216
R-free	0.26800
Structure solution method	MAD
RMSD bond length	0.007
RMSD bond angle	1.700
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.570
High resolution limit [Å]	2.480	2.480
Rmerge	0.064	0.274
Number of reflections	15052
<I/σ(I)>	27.1	4.9
Completeness [%]	98.2	92.8
Redundancy	4.6	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5	293	20% PEG3000 100mM Acetate (pH4.5), VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	4.5	293	20% PEG3000 100mM Acetate (pH4.5), VAPOR DIFFUSION, HANGING DROP, temperature 293K