2YXN

Structual basis of azido-tyrosine recognition by engineered bacterial Tyrosyl-tRNA synthetase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B1
Synchrotron site	SPring-8
Beamline	BL26B1
Temperature [K]	100
Detector technology	CCD
Collection date	2005-07-07
Detector	RIGAKU JUPITER
Wavelength(s)	1.0
Spacegroup name	P 31 2 1
Unit cell lengths	83.155, 83.155, 93.668
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	39.260 - 1.800
R-factor	0.199
Rwork	0.199
R-free	0.24700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1wq3
RMSD bond length	0.011
RMSD bond angle	1.400
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.830
High resolution limit [Å]	1.800	1.800
Rmerge	0.113
Number of reflections	35502
<I/σ(I)>	23	2.08
Completeness [%]	100.0	100
Redundancy	10.8	10.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.5	293	2M ammonium sulfate, 0.1M Hepes, 2% PEG400, pH 7.5, VAPOR DIFFUSION, temperature 293K