2YXF
The high resolution crystal structure of beta2-microglobulin under physiological conditions
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 2005-12-05 |
| Detector | Bruker DIP-6040 |
| Wavelength(s) | 0.65 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 77.644, 28.907, 54.397 |
| Unit cell angles | 90.00, 121.59, 90.00 |
Refinement procedure
| Resolution | 26.490 - 1.130 |
| R-factor | 0.18436 |
| Rwork | 0.184 |
| R-free | 0.19486 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2d4f |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.227 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.630 | 1.190 |
| High resolution limit [Å] | 1.130 | 1.130 |
| Rmerge | 0.051 | 0.332 |
| Number of reflections | 38852 | |
| <I/σ(I)> | 12.4 | 4 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 3.8 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 288 | 25% PEG 4000, 25% Glycerol, 0.06M ammoniumacetate, 0.1M MOPS, pH 7.00, VAPOR DIFFUSION, HANGING DROP, temperature 288K |
