2YWW

Crystal structure of aspartate carbamoyltransferase regulatory chain from Methanocaldococcus jannaschii

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B1
Synchrotron site	SPring-8
Beamline	BL26B1
Temperature [K]	100
Detector technology	CCD
Collection date	2007-03-29
Detector	RIGAKU JUPITER 210
Wavelength(s)	1.282150, 1.20000, 1.00000
Spacegroup name	P 21 21 21
Unit cell lengths	51.003, 71.398, 92.975
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.960 - 2.000
R-factor	0.226
Rwork	0.226
R-free	0.22600
Structure solution method	MAD
RMSD bond length	0.008
RMSD bond angle	3.100
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.070
High resolution limit [Å]	2.000	2.000
Rmerge	0.058	0.211
Number of reflections	23638
Completeness [%]	99.9	100
Redundancy	7	7.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	293	0.16M Ammmounium Acetate, 0.08M Bis-Tris(pH5.5), 20%w/v PEG3350, 10mM ATP, 10mM Zinc chloride, VAPOR DIFFUSION, SITTING DROP, temperature 293K