2YW8
Crystal structure of human RUN and FYVE domain-containing protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-02-05 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.2752 |
| Spacegroup name | P 43 3 2 |
| Unit cell lengths | 115.150, 115.150, 115.150 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.010 - 3.000 |
| R-factor | 0.198 |
| Rwork | 0.198 |
| R-free | 0.22400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1vfy |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.800 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.110 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Number of reflections | 5650 | |
| <I/σ(I)> | 30.2 | 12.5 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 40.9 | 42.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | Bis-tris, Ammonium sulfate, pH6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
