2YVZ
Crystal structure of magnesium transporter MgtE cytosolic domain, Mg2+-free form
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-01-24 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97909, 0.97926, 0.96403 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 77.112, 100.183, 100.158 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.800 - 3.900 |
| R-factor | 0.346 |
| Rwork | 0.346 |
| R-free | 0.37000 |
| Structure solution method | MAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.900 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.970 |
| High resolution limit [Å] | 3.900 | 3.900 |
| Number of reflections | 7435 | |
| <I/σ(I)> | 11.6 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 20% PEG 3350, 0.2M ammonium acetate, 0.5% n-octyl-beta-D-glucopyranoside, 10% ethylene glycol, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
