2YVX

Crystal structure of magnesium transporter MgtE

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	CCD
Collection date	2006-11-28
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97924, 0.97933, 0.98200
Spacegroup name	C 2 2 21
Unit cell lengths	118.314, 134.897, 366.252
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.690 - 3.500
R-factor	0.294
Rwork	0.294
R-free	0.34100
Structure solution method	MAD
RMSD bond length	0.013
RMSD bond angle	1.500
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	CNS (1.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.560
High resolution limit [Å]	3.500	3.500
Number of reflections	37118
<I/σ(I)>	8.6	2.3
Redundancy	16

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	293	2-methyl-2,4-pentanediol (MPD), 40mM magnesium acetate, 100mM MES, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K