2YVW

Crystal structure of UDP-N-acetylglucosamine 1-carboxyvinyltransferase from Aquifex aeolicus VF5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B1
Synchrotron site	SPring-8
Beamline	BL26B1
Temperature [K]	100
Detector technology	CCD
Collection date	2007-03-21
Detector	RIGAKU JUPITER 210
Wavelength(s)	1.0
Spacegroup name	P 62
Unit cell lengths	132.004, 132.004, 58.165
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.640 - 1.810
R-factor	0.195
Rwork	0.195
R-free	0.22000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1uae
RMSD bond length	0.005
RMSD bond angle	1.200
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.870
High resolution limit [Å]	1.810	1.810
Rmerge	0.067	0.329
Number of reflections	52283
<I/σ(I)>	33.47	6.24
Completeness [%]	98.7	90.8
Redundancy	10.4	8.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH	7.4	291	40% (v/v) PEG 400, 0.1M Imidazole, pH 7.4, MICRO BATCH, temperature 291K