2YVS

Crystal structure of glycolate oxidase subunit GlcE from Thermus thermophilus HB8

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B2
Synchrotron site	SPring-8
Beamline	BL26B2
Temperature [K]	100
Detector technology	CCD
Collection date	2005-06-13
Detector	RIGAKU JUPITER 210
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	69.880, 71.690, 89.580
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.547 - 2.000
Rwork	0.208
R-free	0.24930
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	SeMet crystal of Glycolate oxidase subunit GlcE
RMSD bond length	0.006
RMSD bond angle	1.300
Data reduction software	CrystalClear
Data scaling software	CrystalClear
Phasing software	MOLREP
Refinement software	CNS (1.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	55.989
High resolution limit [Å]	2.000
Number of reflections	30844

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.6	298	0.1M Na3 Citrate, 2.2M Na Formate, pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 298K