2YVK

Crystal structure of 5-methylthioribose 1-phosphate isomerase product complex from Bacillus subtilis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44XU
Synchrotron site	SPring-8
Beamline	BL44XU
Temperature [K]	93
Detector technology	CCD
Collection date	2005-05-17
Detector	Bruker DIP-6040
Wavelength(s)	0.9
Spacegroup name	P 1 21 1
Unit cell lengths	79.987, 84.444, 95.299
Unit cell angles	90.00, 92.34, 90.00

Refinement procedure

Resolution	63.160 - 2.400
R-factor	0.212
Rwork	0.212
R-free	0.25700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2yrf
RMSD bond length	0.011
RMSD bond angle	1.500
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	CNS (1.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	94.900
High resolution limit [Å]	2.400
Number of reflections	49797

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.4	293	0.2M Potassium Formate, 15% polyethylene glycol 3350, pH7.4, VAPOR DIFFUSION, SITTING DROP, temperature 293K