2YVG

crystal structure of ferredoxin reductase, BPHA4 (blue-semiquinone)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	100
Detector technology	CCD
Collection date	2005-01-30
Detector	ADSC QUANTUM 315
Spacegroup name	P 61 2 2
Unit cell lengths	98.288, 98.288, 170.066
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	15.000 - 1.600
R-factor	0.195
Rwork	0.194
R-free	0.22000
Structure solution method	DIFFERENCE FOURIER
RMSD bond length	0.012
RMSD bond angle	1.468
Data scaling software	XDS
Refinement software	XTALVIEW

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000	1.680
High resolution limit [Å]	1.600	1.600
Rmerge	0.054	0.641
Number of reflections	63621
<I/σ(I)>	36.5	5.3
Completeness [%]	98.6	97.7
Redundancy	20.8	18.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.4	293	2M SODIUM FORMATE, 0.1M SODIUM ACETATE, pH 5.40, VAPOR DIFFUSION, SITTING DROP, temperature 293K