2YV3
Crystal Structure of Aspartate Semialdehyde Dehydrogenase from Thermus thermophilus HB8
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-10-22 |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 0.9789, 0.9000, 0.9793 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 125.167, 125.167, 116.463 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 2.700 |
| R-factor | 0.303 |
| Rwork | 0.303 |
| R-free | 0.36800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2gz3 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.750 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.071 | 0.294 |
| Number of reflections | 29493 | |
| <I/σ(I)> | 9.2 | 3.1 |
| Completeness [%] | 99.3 | 95.6 |
| Redundancy | 5.2 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 0.1M Tris-HCl (pH 8.2), 0.2M MgCl2, 30% PEG 4000, 3% ethylene glycol, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
