Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-03-15 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 170.610, 222.729, 196.160 |
| Unit cell angles | 90.00, 101.87, 90.00 |
Refinement procedure
| Resolution | 19.980 - 3.400 |
| R-factor | 0.28517 |
| Rwork | 0.283 |
| R-free | 0.34392 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1sfo |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.474 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.500 |
| High resolution limit [Å] | 3.400 | 3.400 |
| Number of reflections | 91438 | |
| <I/σ(I)> | 10.4 | 1.3 |
| Completeness [%] | 93.0 | 76.6 |
| Redundancy | 2.6 | 1.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 200MM NH4OAC, 150MM MG(OAC)2, 50MM HEPES, PH 7.0, 5% PEG6000, pH 7.00, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | NH4OAc | ||
| 2 | 1 | 1 | Mg(OAc)2 | ||
| 3 | 1 | 1 | Hepes | ||
| 4 | 1 | 1 | PEG6000 | ||
| 5 | 1 | 2 | NH4OAc | ||
| 6 | 1 | 2 | Mg(OAc)2 | ||
| 7 | 1 | 2 | Hepes | ||
| 8 | 1 | 2 | PEG6000 |
