2YT4
Crystal structure of human DGCR8 core
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 4A |
| Synchrotron site | PAL/PLS |
| Beamline | 4A |
| Temperature [K] | 113 |
| Detector technology | CCD |
| Collection date | 2005-10-02 |
| Wavelength(s) | 0.98010 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 57.511, 59.985, 70.921 |
| Unit cell angles | 90.00, 106.86, 90.00 |
Refinement procedure
| Resolution | 19.320 - 2.600 |
| R-factor | 0.214 |
| Rwork | 0.214 |
| R-free | 0.26900 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.200 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 8118 | |
| <I/σ(I)> | 19.7 | 2.2 |
| Redundancy | 4.4 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | 1.8M sodium/potassium phosphate buffer pH8.0, 0.9mM CTAB (Cetyltrimethylammonium Bromide), 5mM DTT, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
