2YQ1

MHV-68 LANA (ORF73) C-terminal domain: triclinic crystal form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.2
Synchrotron site	BESSY
Beamline	14.2
Temperature [K]	100
Detector technology	CCD
Collection date	2012-07-05
Detector	MARRESEARCH
Spacegroup name	P 1
Unit cell lengths	35.958, 53.395, 70.859
Unit cell angles	106.00, 93.47, 109.80

Refinement procedure

Resolution	24.062 - 2.300
R-factor	0.2024
Rwork	0.200
R-free	0.24560
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	1.009
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.270
High resolution limit [Å]	2.140	2.140
Rmerge	0.100	0.380
Number of reflections	24125
<I/σ(I)>	7.4	2.34
Completeness [%]	92.8	80.8
Redundancy	2.03	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9	293	1.5 UL OF 0.3 MM PROTEIN IN 5 MM BISTRIS, PH 6.5, 5 MM DTT WERE ADDED TO 1.5 UL OF 2.0 M SODIUM FORMATE, 15% (W/V) PEG 6000. THE MIXTURE WAS INCUBATED AT 20 DEGREE CENTIGRADE IN A HANGING DROP SETUP. CRYSTALS GREW IN A FEW DAYS AND WERE CRYO-PROTECTED BY SHORT SOAKING IN 2.0 M SODIUM FORMATE, 15% (W/V) PEG 6000, 25% (V/V) GLYCEROL.