2YQ0

KSHV LANA (ORF73) C-terminal domain, octameric ring: cubic crystal form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.2
Synchrotron site	BESSY
Beamline	14.2
Temperature [K]	100
Detector technology	CCD
Collection date	2012-07-05
Detector	MARRESEARCH
Spacegroup name	P 4 3 2
Unit cell lengths	107.400, 107.400, 107.400
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.963 - 3.911
R-factor	0.2623
Rwork	0.260
R-free	0.31660
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2ypy
RMSD bond length	0.003
RMSD bond angle	0.746
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	4.010
High resolution limit [Å]	3.910	3.910
Rmerge	0.110	0.730
Number of reflections	2178
<I/σ(I)>	21.73	3.92
Completeness [%]	99.7	100
Redundancy	10.8	11.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	2.5	277	1 UL OF 1 MM PROTEIN IN 5 MM BISTRIS, PH 6.5, 2 MM DTT WAS ADDED TO 1 UL OF 0.1 M SODIUM CITRATE, PH 2.5, 1.5 M AMMONIUM SULFATE. THE MIXTURE WAS INCUBATED AT 4 DEGREE CENTIGRADE IN A HANGING DROP SETUP. CRYSTALS GREW IN A FEW DAYS AND WERE CRYO-PROTECTED BY SHORT SOAKING IN 0.1 M SODIUM CITRATE, PH 2.5, 1.5 M AMMONIUM SULFATE, 25% (V/V) GLYCEROL.