2YPJ
Non-catalytic carbohydrate binding module CBM65B
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-07-14 |
| Detector | ADSC CCD |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 57.920, 57.920, 116.740 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 51.880 - 2.350 |
| R-factor | 0.22706 |
| Rwork | 0.224 |
| R-free | 0.28035 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ba6 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.556 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 58.370 | 2.480 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.080 | 0.660 |
| Number of reflections | 8856 | |
| <I/σ(I)> | 13.4 | 2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 6.2 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 200 AMMONIUM ACETATE, 100 MM TRI-SODIUM CITRATE PH 5.6, 30% PEG 4000 |
