2YOQ
Structure of FAM3B PANDER E30 construct
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-11-11 |
| Detector | ADSC CCD |
| Spacegroup name | P 31 |
| Unit cell lengths | 86.540, 86.540, 96.280 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 21.640 - 2.350 |
| R-factor | 0.1599 |
| Rwork | 0.158 |
| R-free | 0.18970 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.160 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.470 | 2.430 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.130 | 0.790 |
| Number of reflections | 33513 | |
| <I/σ(I)> | 11.2 | 2.6 |
| Completeness [%] | 99.9 | 99.7 |
| Redundancy | 4.3 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | pH 7 |
