2YOE

X-ray structure of a pentameric ligand gated ion channel from Erwinia chrysanthemi (ELIC) in complex with GABA and flurazepam

Replaces:  4A96

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	CCD
Collection date	2011-05-21
Detector	ADSC QUANTUM 315r
Spacegroup name	P 1 21 1
Unit cell lengths	105.771, 266.512, 110.982
Unit cell angles	90.00, 108.82, 90.00

Refinement procedure

Resolution	25.028 - 3.900
R-factor	0.1875
Rwork	0.185
R-free	0.22990
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2vl0
RMSD bond length	0.005
RMSD bond angle	1.099
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall
Low resolution limit [Å]	24.980
High resolution limit [Å]	3.800
Rmerge	0.120
Number of reflections	54291
<I/σ(I)>	8
Completeness [%]	95.7
Redundancy	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1