2YN6
Pentameric Ligand-Gated Ion Channel ELIC in Complex with Barium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-03-30 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 43 |
| Unit cell lengths | 100.473, 100.473, 263.951 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.986 - 3.310 |
| R-factor | 0.2285 |
| Rwork | 0.227 |
| R-free | 0.25780 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vl0 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.218 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.500 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Rmerge | 0.120 | 0.640 |
| Number of reflections | 38612 | |
| <I/σ(I)> | 12.1 | 2.7 |
| Completeness [%] | 99.4 | 96.6 |
| Redundancy | 6.5 | 6.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 50 MM ADA PH6.5, 50 MM BAAC2, 10% PEG4000 |
