2YMS

Structure and assembly of a b-propeller with nine blades and a new conserved repetitive sequence motif

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Detector	DECTRIS PILATUS
Spacegroup name	C 1 2 1
Unit cell lengths	88.570, 83.450, 57.370
Unit cell angles	90.00, 118.94, 90.00

Refinement procedure

Resolution	50.206 - 2.101
R-factor	0.1899
Rwork	0.187
R-free	0.23950
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.069
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX ((PHENIX.REFINE: 1.8.1_1168))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.200
High resolution limit [Å]	2.100	2.100
Rmerge	0.060	0.450
Number of reflections	21022
<I/σ(I)>	7.4	1.6
Completeness [%]	93.4	91.2
Redundancy	2.1	2.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1