2YLB
Structure of Salmonella typhimurium Hfq at 1.15 A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 2009-05-19 |
| Detector | MARRESEARCH |
| Spacegroup name | P 61 |
| Unit cell lengths | 61.400, 61.400, 167.030 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 18.840 - 1.150 |
| R-factor | 0.1701 |
| Rwork | 0.168 |
| R-free | 0.20760 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1hk9 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.614 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.180 |
| High resolution limit [Å] | 1.150 | 1.150 |
| Number of reflections | 123284 | |
| <I/σ(I)> | 12.8 | 2.52 |
| Completeness [%] | 98.0 | 96.9 |
| Redundancy | 4.9 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 0.1 M HEPES (PH=7.0), 0.5 % JEFFAMINE, 1.1 M MALONATE |
