2YL9
INHIBITION OF THE PNEUMOCOCCAL VIRULENCE FACTOR STRH AND MOLECULAR INSIGHTS INTO N-GLYCAN RECOGNITION AND HYDROLYSIS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARMOSAIC 325 mm CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 67.270, 115.890, 132.350 |
| Unit cell angles | 90.00, 99.46, 90.00 |
Refinement procedure
| Resolution | 40.740 - 2.650 |
| R-factor | 0.23257 |
| Rwork | 0.229 |
| R-free | 0.29848 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.929 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.610 | 2.790 |
| High resolution limit [Å] | 2.650 | 2.650 |
| Rmerge | 0.140 | 0.390 |
| Number of reflections | 55642 | |
| <I/σ(I)> | 8.5 | 3.5 |
| Completeness [%] | 95.4 | 88.9 |
| Redundancy | 4.8 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
