2YL8
Inhibition of the pneumococcal virulence factor StrH and molecular insights into N-glycan recognition and hydrolysis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARMOSAIC 325 mm CCD |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 78.570, 109.290, 112.930 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.600 - 1.750 |
| R-factor | 0.16647 |
| Rwork | 0.164 |
| R-free | 0.20522 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.425 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.600 | 1.840 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.090 | 0.400 |
| Number of reflections | 49280 | |
| <I/σ(I)> | 20.4 | 4.4 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 8 | 8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
