2YKT
Crystal structure of the I-BAR domain of IRSp53 (BAIAP2) in complex with an EHEC derived Tir peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-08-21 |
| Detector | MARRESEARCH |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 88.354, 187.362, 36.806 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 9.440 - 2.110 |
| R-factor | 0.23559 |
| Rwork | 0.233 |
| R-free | 0.27604 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1y2o |
| RMSD bond length | 0.022 |
| RMSD bond angle | 1.901 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 9.440 | 2.160 |
| High resolution limit [Å] | 2.110 | 2.110 |
| Rmerge | 0.080 | 0.530 |
| Number of reflections | 18190 | |
| <I/σ(I)> | 20.13 | 3.13 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 12.7 | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 16% (W/V) PEG 3350, 0.3M AMMONIUM SULPHATE |
