2YKP
Structure of the human LINE-1 ORF1p trimer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 2009-05-19 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 73.730, 89.920, 111.620 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 61.520 - 3.100 |
| R-factor | 0.2275 |
| Rwork | 0.225 |
| R-free | 0.27360 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 2YKO AND 2W7A |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.484 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 61.500 | 3.180 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Number of reflections | 13700 | |
| <I/σ(I)> | 10.8 | 1.7 |
| Completeness [%] | 97.9 | 99.6 |
| Redundancy | 2.4 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 200 MM K-CITRATE, 20 % PEG 3350 |
