2YJZ
Rat STEAP4 oxidoreductase domain complexed with NADP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-01-18 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 69.228, 85.111, 128.035 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.000 - 2.200 |
| R-factor | 0.20471 |
| Rwork | 0.203 |
| R-free | 0.23784 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vq3 |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.861 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MrBUMP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.100 | 0.460 |
| Number of reflections | 39044 | |
| <I/σ(I)> | 7.9 | 2.8 |
| Completeness [%] | 99.1 | 94.8 |
| Redundancy | 7.8 | 6.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.5 | 0.1 M BIS-TRIS HCL PH 5.5; 2.1 M AMMONIUM SULFATE; 0.001M NADPH. |
