2YJY
A specific and modular binding code for cytosine recognition in PUF domains
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 287 |
Detector technology | CCD |
Collection date | 2011-03-12 |
Detector | MARRESEARCH MX-300 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 35.796, 59.340, 344.724 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 24.977 - 2.598 |
R-factor | 0.2153 |
Rwork | 0.210 |
R-free | 0.30560 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1m8y |
RMSD bond length | 0.006 |
RMSD bond angle | 1.092 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 24.980 | 2.640 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.130 | 0.270 |
Number of reflections | 20661 | |
<I/σ(I)> | 9 | 3.4 |
Completeness [%] | 86.7 | 79.2 |
Redundancy | 3.9 | 2.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6 | 30% PEG 3350, 0.2M SODIUM TARTRATE, 0.1M BIS-TRIS PH 6.0 |