2YJK
Structure of Dps from MICROBACTERIUM ARBORESCENS in the high iron form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 87.580, 92.050, 128.580 |
| Unit cell angles | 90.00, 96.04, 90.00 |
Refinement procedure
| Resolution | 28.980 - 2.000 |
| R-factor | 0.17227 |
| Rwork | 0.170 |
| R-free | 0.21650 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1o9r |
| RMSD bond length | 0.025 |
| RMSD bond angle | 1.821 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.100 | 0.880 |
| Number of reflections | 129605 | |
| <I/σ(I)> | 7.3 | 1.5 |
| Completeness [%] | 98.0 | 96.8 |
| Redundancy | 2.05 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 18% PEG 8000, 2% ISO-PROPANOL, 0.1 M NA-ACETATE, PH 7.5. |
