2YJF

Oligomeric assembly of actin bound to MRTF-A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	CCD
Collection date	2009-02-08
Detector	ADSC CCD
Spacegroup name	P 65 2 2
Unit cell lengths	182.910, 182.910, 378.281
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.525 - 3.500
R-factor	0.2396
Rwork	0.238
R-free	0.27220
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2v52
RMSD bond length	0.011
RMSD bond angle	1.023
Phasing software	PHENIX
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.000	3.690
High resolution limit [Å]	3.500	3.500
Rmerge	0.090	0.390
Number of reflections	47726
<I/σ(I)>	7.1	2
Completeness [%]	99.6	99.6
Redundancy	5.3	5.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		5.3		pH 5.3