2YIU
X-ray structure of the dimeric cytochrome BC1 complex from the soil bacterium paracoccus denitrificans at 2.7 angstrom resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-08-08 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 85.300, 164.910, 100.610 |
| Unit cell angles | 90.00, 103.18, 90.00 |
Refinement procedure
| Resolution | 97.960 - 2.700 |
| R-factor | 0.24033 |
| Rwork | 0.238 |
| R-free | 0.29049 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2qjy |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.761 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0066) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 54.970 | 2.850 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.130 | 0.320 |
| Number of reflections | 70055 | |
| <I/σ(I)> | 3.7 | 2.2 |
| Completeness [%] | 95.0 | 90 |
| Redundancy | 3.1 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.6 | 100 MM SODIUM ACETATE, PH 4.6, 50 MM NACL, 30% 2-METHYL 2,4-PENTANDIOL (MPD) |
