2YIA

Structure of the RNA polymerase VP1 from Infectious Pancreatic Necrosis Virus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I02
Synchrotron site	Diamond
Beamline	I02
Temperature [K]	100
Detector technology	CCD
Collection date	2009-05-18
Detector	ADSC CCD
Spacegroup name	P 21 21 21
Unit cell lengths	200.938, 209.467, 243.393
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	145.010 - 3.020
R-factor	0.1877
Rwork	0.187
R-free	0.21640
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2yi9
RMSD bond length	0.011
RMSD bond angle	1.130
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	BUSTER (2.9.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	145.010	3.100
High resolution limit [Å]	3.020	3.020
Rmerge	0.160	0.860
Number of reflections	200159
<I/σ(I)>	8.6	2
Completeness [%]	100.0	100
Redundancy	4.3	4.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP			CRYSTALS WERE GROWN IN SITTING DROPS CONTAINING 200 NL PROTEIN (5.8-6.8 MG/ML) AND 100 NL RESERVOIR SOLUTION (20-18% W/V PEG3350, 0.10-0.09 M BIS-TRIS PROPANE PH 7.5, 0.2-0.18 M SODIUM CITRATE) EQUILIBRATED AGAINST 95 UL RESERVOIRS AT 20. C. CRYSTALS WERE CRYOPROTECTED BY SOAKING IN MOTHER-LIQUOR SUPPLEMENTED WITH 20-25% GLYCEROL FOR 1-10 MIN.