2YHC

Structure of BamD from E. coli

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Detector	DECTRIS PILATUS 6M
Spacegroup name	P 1 21 1
Unit cell lengths	53.112, 33.428, 57.783
Unit cell angles	90.00, 111.41, 90.00

Refinement procedure

Resolution	28.390 - 1.800
R-factor	0.20007
Rwork	0.198
R-free	0.23262
Structure solution method	MIRAS
Starting model (for MR)	NONE
RMSD bond length	0.024
RMSD bond angle	2.095
Data reduction software	XDS
Data scaling software	XDS
Phasing software	SHARP
Refinement software	REFMAC (5.5.0071)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	1.900
High resolution limit [Å]	1.800	1.800
Rmerge	0.040	0.510
Number of reflections	16896
<I/σ(I)>	20.3	3.9
Completeness [%]	99.4	97.8
Redundancy	6.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7		30% PEG 4000, 10% I-PROH, 0.1 M HEPES PH 7.5 .