2YH5
Structure of the C-terminal domain of BamC
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 29.740, 59.120, 31.060 |
| Unit cell angles | 90.00, 116.37, 90.00 |
Refinement procedure
| Resolution | 29.560 - 1.250 |
| R-factor | 0.14828 |
| Rwork | 0.146 |
| R-free | 0.18387 |
| Structure solution method | MIRAS |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.029 |
| RMSD bond angle | 2.157 |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 1.310 | 1.310 |
| High resolution limit [Å] | 1.280 | 1.280 |
| Rmerge | 0.060 | 0.570 |
| Number of reflections | 25297 | |
| <I/σ(I)> | 10.3 | 2.1 |
| Completeness [%] | 98.4 | 95.6 |
| Redundancy | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 25% PEG1000, 0.1 M MES PH 6.5 |
