2YGY
Structure of wild type E. coli N-acetylneuraminic acid lyase in space group P21 crystal form II
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-04-16 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 84.260, 95.870, 91.380 |
| Unit cell angles | 90.00, 115.33, 90.00 |
Refinement procedure
| Resolution | 82.590 - 1.900 |
| R-factor | 0.196 |
| Rwork | 0.194 |
| R-free | 0.22400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2wnn |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.165 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0105) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.940 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.100 | 0.320 |
| Number of reflections | 102699 | |
| <I/σ(I)> | 7 | 3.7 |
| Completeness [%] | 99.4 | 99.5 |
| Redundancy | 3.1 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 100MM TRIS-HCL PH 8.0, 200MM NACL, 18% PEG3350 |
