2YGX
Structure of the mixed-function P450 MycG in P21 space group
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 2011-04-15 |
Detector | MARRESEARCH |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 57.150, 161.980, 100.110 |
Unit cell angles | 90.00, 89.99, 90.00 |
Refinement procedure
Resolution | 80.990 - 2.390 |
R-factor | 0.18733 |
Rwork | 0.184 |
R-free | 0.24749 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2y98 |
RMSD bond length | 0.017 |
RMSD bond angle | 1.715 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 161.980 | 2.520 |
High resolution limit [Å] | 2.390 | 2.390 |
Rmerge | 0.070 | 0.300 |
Number of reflections | 68714 | |
<I/σ(I)> | 10.8 | 3.2 |
Completeness [%] | 95.6 | 76.6 |
Redundancy | 3.8 | 2.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7 | 0.6M SUCCINIC ACID PH 7, 2% GLYCEROL, 0.0075MM CYMAL-7 |