2YGV
Conserved N-terminal domain of the yeast Histone Chaperone Asf1 in complex with the C-terminal fragment of Rad53
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 287 |
| Detector technology | CCD |
| Collection date | 2009-11-08 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 125.240, 98.180, 86.490 |
| Unit cell angles | 90.00, 132.49, 90.00 |
Refinement procedure
| Resolution | 38.900 - 2.940 |
| R-factor | 0.1908 |
| Rwork | 0.188 |
| R-free | 0.25700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1roc |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.330 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | BUSTER (2.8.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.900 | 3.120 |
| High resolution limit [Å] | 2.940 | 2.940 |
| Rmerge | 0.020 | 0.090 |
| Number of reflections | 16378 | |
| <I/σ(I)> | 10.59 | 2.36 |
| Completeness [%] | 98.7 | 97.5 |
| Redundancy | 3.96 | 3.82 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | 5% PEG 8000, 0.1M NACACO PH 6, 0.2M MG ACETATE |
