2YGS

CARD DOMAIN FROM APAF-1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Spacegroup name	C 1 2 1
Unit cell lengths	56.700, 36.000, 47.900
Unit cell angles	90.00, 118.20, 90.00

Refinement procedure

Resolution	8.000 - 1.600
Rwork	0.198
R-free	0.23600
Structure solution method	MIR
RMSD bond length	0.006
RMSD bond angle	1.275
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR
Refinement software	X-PLOR (3.8)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.660
High resolution limit [Å]	1.600	1.600
Rmerge	0.057 *	0.120 *
Total number of observations	92435 *
Number of reflections	11042 *
Completeness [%]	96.8 *	88.8 *
Redundancy	11

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	4.6 *	22 *	pH 6.0

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	reservoir	acetate	100 (mM)
3	1	reservoir	PEG4000	24-28 (%(w/w))
4	1	reservoir	ammonium acetate	200 (mM)
5	1	reservoir	dithiothreitol	10 (mM)