2YGF
L89V, L93I and V136M Mutant of N-Term HSP90 complexed with Geldanamycin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-11-10 |
| Detector | RIGAKU CCD |
| Spacegroup name | P 43 2 2 |
| Unit cell lengths | 74.121, 74.121, 110.433 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.872 - 2.000 |
| R-factor | 0.2164 |
| Rwork | 0.214 |
| R-free | 0.26370 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | IN-HOUSE MODEL |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.089 |
| Data reduction software | CrystalClear |
| Data scaling software | CrystalClear |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 74.120 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.120 | 0.730 |
| Number of reflections | 21531 | |
| <I/σ(I)> | 22.3 | 3.8 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 43.3 | 28.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | pH 7 |
