2YGC
Structure of vaccinia virus D13 scaffolding protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-12-14 |
| Detector | ADSC CCD |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 190.580, 190.580, 252.960 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 39.280 - 3.020 |
| R-factor | 0.1717 |
| Rwork | 0.170 |
| R-free | 0.20290 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.100 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | autoSHARP |
| Refinement software | BUSTER (2.11.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.300 | 3.100 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.210 | 1.500 |
| Number of reflections | 53583 | |
| <I/σ(I)> | 16.9 | 2 |
| Completeness [%] | 99.7 | 98.6 |
| Redundancy | 20.8 | 15.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 4.0 M SODIUM FORMATE., pH 7.5 |
