2YFS
Crystal structure of inulosucrase from Lactobacillus johnsonii NCC533 in complex with sucrose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2010-11-22 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | I 4 2 2 |
Unit cell lengths | 171.951, 171.951, 116.503 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.450 - 2.600 |
R-factor | 0.18196 |
Rwork | 0.180 |
R-free | 0.22397 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1oyg |
RMSD bond length | 0.012 |
RMSD bond angle | 1.394 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.750 | 2.740 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.100 | 0.590 |
Number of reflections | 27086 | |
<I/σ(I)> | 12.6 | 3.7 |
Completeness [%] | 100.0 | 100 |
Redundancy | 7.5 | 7.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6 | 2M (NH4)2)SO4, 5% (V/V) 2-PROPANOL, pH 6 |