2YDY

Crystal structure of human S-adenosylmethionine synthetase 2, beta subunit in Orthorhombic crystal form

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I02
Synchrotron site	Diamond
Beamline	I02
Temperature [K]	100
Detector technology	CCD
Collection date	2010-12-09
Detector	ADSC CCD
Spacegroup name	C 2 2 21
Unit cell lengths	40.980, 111.770, 123.120
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.070 - 2.250
R-factor	0.1773
Rwork	0.175
R-free	0.21750
Structure solution method	MAD
Starting model (for MR)	NONE
RMSD bond length	0.009
RMSD bond angle	0.970
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	SHARP
Refinement software	BUSTER (2.8.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	61.610	2.290
High resolution limit [Å]	2.170	2.170
Rmerge	0.050	0.910
Number of reflections	15419
<I/σ(I)>	9.7	2.4
Completeness [%]	99.8	98.9
Redundancy	6.6	6.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1