2YDS
CpOGA D298N in complex with TAB1-derived O-GlcNAc peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-08-07 |
| Detector | ADSC CCD |
| Spacegroup name | P 61 |
| Unit cell lengths | 118.090, 118.090, 148.260 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 25.000 - 2.550 |
| R-factor | 0.20087 |
| Rwork | 0.200 |
| R-free | 0.23846 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2j62 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.404 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0088) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 25.000 |
| High resolution limit [Å] | 2.550 |
| Rmerge | 0.120 |
| Number of reflections | 37698 |
| <I/σ(I)> | 7.1 |
| Completeness [%] | 99.0 |
| Redundancy | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | pH 8.5 |
