2YDG
Ascorbate co-crystallized HEWL.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-05-10 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.939 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 78.770, 78.770, 37.370 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.790 - 2.000 |
| R-factor | 0.15501 |
| Rwork | 0.152 |
| R-free | 0.18648 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2w1l |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.689 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 2.440 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.050 | 0.070 |
| Number of reflections | 8387 | 3693 |
| <I/σ(I)> | 8.7 | 8.6 |
| Completeness [%] | 99.8 | 99.9 |
| Redundancy | 6.6 | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.7 | 293 | 200 MM SODIUM ACETATE BUFFER AT PH 4.7 CONTAINING 10 % W/V NACL. |
