2YDC
Catalytic domain of mouse 2',3'-cyclic nucleotide 3'- phosphodiesterase, soaked with GTP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MAX II BEAMLINE I911-2 |
Synchrotron site | MAX II |
Beamline | I911-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-05-15 |
Detector | MARRESEARCH MAR165 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 41.890, 46.430, 106.490 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.946 - 2.050 |
R-factor | 0.2041 |
Rwork | 0.202 |
R-free | 0.24830 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2xmi |
RMSD bond length | 0.003 |
RMSD bond angle | 0.762 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 2.100 |
High resolution limit [Å] | 2.050 | 2.050 |
Rmerge | 0.080 | 1.080 |
Number of reflections | 13618 | |
<I/σ(I)> | 15.66 | 2.18 |
Completeness [%] | 99.9 | 100 |
Redundancy | 6.9 | 6.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 3.5 | CRYSTALLIZATION: 50 MM NA-CITRATE PH 3.5 & 30 % PEG 3000, SOAKING: 50 MM NA-CITRATE PH 3.5, 35 % PEG 1500 & 20 MM GTP FOR 3 H |