2YCY
TURKEY BETA1 ADRENERGIC RECEPTOR WITH STABILISING MUTATIONS AND BOUND ANTAGONIST CYANOPINDOLOL
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2007-07-01 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 184.760, 56.161, 89.778 |
| Unit cell angles | 90.00, 111.27, 90.00 |
Refinement procedure
| Resolution | 47.510 - 3.150 |
| R-factor | 0.25536 |
| Rwork | 0.254 |
| R-free | 0.29174 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vt4 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.002 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.500 | 3.290 |
| High resolution limit [Å] | 3.150 | 3.150 |
| Rmerge | 0.140 | 0.350 |
| Number of reflections | 12442 | |
| <I/σ(I)> | 6.5 | 2 |
| Completeness [%] | 83.0 | 53 |
| Redundancy | 2.5 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.2 | pH 7.2 |
