2YCK

methyltransferase bound with tetrahydrofolate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.2
Synchrotron site	BESSY
Beamline	14.2
Temperature [K]	100
Detector technology	CCD
Detector	MARMOSAIC 225 mm CCD
Spacegroup name	P 43 21 2
Unit cell lengths	63.955, 63.955, 175.095
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.187 - 2.150
R-factor	0.1803
Rwork	0.178
R-free	0.23030
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.055
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.000	2.210
High resolution limit [Å]	2.150	2.150
Rmerge	0.050	0.570
Number of reflections	20410
<I/σ(I)>	18.45	2.59
Completeness [%]	98.9	99.5
Redundancy	3.9	3.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1